
Ozone Therapy Course
Expand your nursing practice with a comprehensive foundation in ozone therapy — from biochemistry and equipment operation to advanced clinical protocols. This course equips you with the practical skills and safety knowledge to deliver ozone treatments confidently across a wide range of patient conditions. Step into a growing field of integrative medicine and set yourself apart as a clinically competent ozone therapy nurse.
What you will learn:
This course covers the science, safety, and clinical application of ozone therapy from the ground up. You will learn how ozone interacts with the body, how to operate and maintain generation equipment, and how to apply systemic and local delivery methods correctly. You will develop structured patient assessment and care planning skills, and study condition-specific protocols for wounds, circulatory disorders, musculoskeletal pain, and more. The course also addresses documentation, legal accountability, and professional standards. By the end, you will have the knowledge and procedural competency to practise ozone therapy safely and effectively.
How you study in practice Ozone Therapy Course
How you practise Ozone Therapy Course
For companies looking to train their teams
With Dedika for businesses, the course includes exercises and examples tailored to your company and its specific needs.
Course content
8 Chapters • 40 LessonsDuration between 4 and 360 hours (you decide)
Chapter 1HideHide detailsSee detailsFoundations of Ozone Therapy
Foundations of Ozone Therapy
Lesson 1 • Ozone Chemistry and Properties
Covers ozone's molecular structure, reactivity, and stability in medical-grade contexts. Provides the chemical foundation required to understand therapeutic and safety principles.
Lesson 2 • History of Ozone in Medicine
Traces ozone therapy from early 20th-century use to modern clinical practice. Contextualises current protocols within a historical and evidence-based framework.
Lesson 3 • Regulatory and Ethical Framework
Outlines the professional, ethical, and regulatory landscape governing ozone therapy practice. Prepares nurses to operate within sanctioned boundaries and informed-consent standards.
Lesson 4 • Therapeutic Indications Overview
Introduces the primary conditions addressed by ozone therapy across medical specialties. Sets the scope of practice nurses will encounter in clinical settings.
Lesson 5 • Biological Mechanisms of Ozone
Explains how ozone interacts with blood, tissues, and cellular pathways to produce therapeutic effects. Links biochemistry to observable clinical outcomes.
Chapter 2HideHide detailsSee detailsOzone Generation and Equipment
Ozone Generation and Equipment
Lesson 1 • Types of Ozone Generators
Compares corona discharge, ultraviolet, and cold plasma generator technologies. Enables nurses to identify appropriate equipment for specific clinical applications.
Lesson 2 • Oxygen Supply and Gas Purity
Covers medical-grade oxygen requirements, cylinder handling, and purity verification. Proper oxygen sourcing directly determines the quality and safety of generated ozone.
Lesson 3 • Ozone Concentration and Dosimetry
Explains how ozone concentration is measured in micrograms per millilitre and how dose is calculated. Accurate dosimetry is essential for therapeutic efficacy and patient safety.
Lesson 4 • Equipment Setup and Calibration
Provides step-by-step procedures for assembling, calibrating, and verifying ozone delivery systems. Ensures nurses can prepare equipment reliably before each patient session.
Lesson 5 • Equipment Maintenance and Troubleshooting
Addresses routine maintenance schedules, component replacement, and common equipment faults. Minimises downtime and ensures consistent ozone output across clinical sessions.
Chapter 3HideHide detailsSee detailsSafety, Hazards, and Infection Control
Safety, Hazards, and Infection Control
Lesson 1 • Personal Protective Equipment
Specifies PPE requirements for ozone therapy procedures, including gloves, eye protection, and respiratory precautions. Correct PPE selection prevents occupational exposure during all delivery methods.
Lesson 2 • Infection Control in Ozone Procedures
Applies standard and transmission-based precautions to ozone therapy settings, including sterile technique for invasive routes. Prevents healthcare-associated infections during all ozone interventions.
Lesson 3 • Ozone Toxicology and Exposure Limits
Details ozone's toxic effects on the respiratory system and established occupational exposure thresholds. Nurses learn to recognise overexposure signs and apply preventive measures.
Lesson 4 • Ventilation and Environmental Controls
Explains room ventilation requirements, ozone scavengers, and ambient monitoring to maintain safe air quality. Environmental controls are the primary defence against airborne ozone accumulation.
Lesson 5 • Contraindications and Patient Screening
Identifies absolute and relative contraindications, including G6PD deficiency, hyperthyroidism, and pregnancy. Systematic screening prevents adverse events before therapy begins.
Chapter 4HideHide detailsSee detailsSystemic Ozone Delivery Methods
Systemic Ozone Delivery Methods
Lesson 1 • Minor Autohemotherapy Protocol
Covers the technique for ozonating a small blood volume and administering it intramuscularly. Contrasts minor AHT with MAH in terms of dose, indication, and patient experience.
Lesson 2 • MAH Procedure Step by Step
Provides a detailed procedural protocol for blood withdrawal, ozonation, and reinfusion in MAH. Precise technique ensures therapeutic efficacy and minimises procedural complications.
Lesson 3 • Major Autohemotherapy Principles
Explains the physiological rationale and clinical indications for major autohemotherapy (MAH). Establishes the conceptual basis before procedural steps are introduced.
Lesson 4 • Intravenous Ozone Saline Infusion
Describes the preparation of ozonated saline and its intravenous administration for systemic effects. Addresses concentration limits and infusion rate controls to prevent gas embolism.
Lesson 5 • Rectal Insufflation as Systemic Route
Presents rectal insufflation as a non-invasive systemic delivery alternative with documented absorption. Covers patient preparation, volume parameters, and post-procedure care.
Chapter 5HideHide detailsSee detailsLocal and Topical Ozone Applications
Local and Topical Ozone Applications
Lesson 1 • Ozone Water in Local Care
Describes the preparation and use of ozonated water for wound irrigation, oral rinsing, and surface disinfection. Ozonated water extends topical ozone benefits to fluid-based clinical applications.
Lesson 2 • Ear Insufflation Technique
Presents auricular ozone insufflation for local ear conditions and its systemic reflex effects. Covers equipment, flow rates, and contraindications specific to the ear canal.
Lesson 3 • Ozonated Oils and Topical Preparations
Covers the preparation, storage, and clinical application of ozonated olive and sunflower oils. Topical ozone preparations are foundational to wound and dermatological care protocols.
Lesson 4 • Vaginal and Bladder Insufflation
Details the protocols for vaginal and bladder ozone insufflation in gynaecological and urological conditions. Emphasises patient dignity, sterile technique, and anatomical safety considerations.
Lesson 5 • Wound Bagging and Limb Insufflation
Explains the technique of enclosing a wound or limb in a sealed bag and delivering ozone gas directly. Addresses bag selection, gas flow rates, and treatment duration for optimal wound outcomes.
Chapter 6HideHide detailsSee detailsPatient Assessment and Care Planning
Patient Assessment and Care Planning
Lesson 1 • Outcome Evaluation and Plan Adjustment
Covers tools for measuring therapeutic progress, including validated scales and biomarker trends. Outcome data drives evidence-based adjustments to the ongoing ozone treatment plan.
Lesson 2 • Individualised Ozone Treatment Planning
Guides nurses in selecting delivery routes, concentrations, and session frequency based on patient data. Individualised planning aligns ozone parameters with therapeutic goals and patient tolerance.
Lesson 3 • Comprehensive Pre-Therapy Assessment
Establishes a structured assessment framework covering medical history, laboratory values, and functional status. Thorough pre-therapy evaluation is the foundation of individualised ozone care planning.
Lesson 4 • Monitoring During Ozone Sessions
Defines intra-session monitoring parameters, including vital signs, patient-reported symptoms, and visual observation. Continuous monitoring enables early detection of adverse reactions during treatment.
Lesson 5 • Adverse Reaction Recognition and Response
Identifies common and serious adverse reactions, from Herxheimer responses to vasovagal episodes. Nurses learn structured response protocols to manage reactions safely and promptly.
Chapter 7HideHide detailsSee detailsClinical Applications by Condition
Clinical Applications by Condition
Lesson 1 • Circulatory and Metabolic Conditions
Presents ozone protocols for peripheral arterial disease, diabetes-related complications, and metabolic syndrome. Systemic delivery methods are prioritised to improve tissue oxygenation and metabolic markers.
Lesson 2 • Neurological and Pain Syndromes
Explores ozone therapy's role in neuropathic pain, fibromyalgia, and post-viral fatigue syndromes. Nurses coordinate care plans that combine ozone with pain management and functional rehabilitation.
Lesson 3 • Chronic Wound and Ulcer Management
Applies ozone delivery methods to diabetic foot ulcers, pressure injuries, and venous leg ulcers. Integrates wound staging, ozone selection, and dressing protocols into a unified care approach.
Lesson 4 • Infectious and Immune Conditions
Addresses ozone therapy protocols for chronic infections, viral conditions, and immune dysregulation. Nurses apply systemic and local methods within a multidisciplinary infection management framework.
Lesson 5 • Musculoskeletal Pain Conditions
Covers ozone injection and insufflation protocols for joint pain, herniated discs, and tendinopathies. Nurses support physicians in procedural preparation, patient positioning, and post-injection monitoring.
Chapter 8HideHide detailsSee detailsDocumentation, Quality, and Professional Practice
Documentation, Quality, and Professional Practice
Lesson 1 • Legal Accountability and Risk Management
Addresses nursing liability, incident reporting, and risk mitigation strategies specific to ozone therapy. Nurses learn to identify and reduce legal and clinical risks in their daily practice.
Lesson 2 • Clinical Documentation Standards
Defines required documentation elements for ozone therapy sessions, including dose, route, response, and adverse events. Accurate records support continuity of care, legal compliance, and quality review.
Lesson 3 • Professional Development and Competency
Outlines pathways for maintaining and advancing ozone therapy competency through continuing education and peer review. Professional development sustains safe practice as evidence and technology evolve.
Lesson 4 • Informed Consent and Patient Education
Covers the elements of valid informed consent and effective patient education strategies for ozone therapy. Nurses ensure patients make autonomous, well-informed decisions about their treatment.
Lesson 5 • Quality Assurance in Ozone Practice
Introduces quality assurance frameworks, audit cycles, and key performance indicators for ozone therapy services. Systematic quality processes ensure consistent, safe, and effective patient outcomes.
Your valid completion certificate
This course is for you:
Registered nurses: seeking to add a specialized integrative therapy to their practice.
Wound care nurses: looking for advanced adjunct tools for complex, hard-to-heal cases.
Clinic nurses: wanting to support physicians offering ozone-based treatment programs.
Holistic health nurses: ready to formalize their integrative practice with clinical protocols.
Nurse entrepreneurs: planning to launch or expand an independent ozone therapy service.
Chronic disease nurses: aiming to broaden options for patients with long-term conditions.
What our students say
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