ATP Course

Lesson 1 • Enzymes and Coenzymes in ATP Synthesis

Identifies key enzymes and cofactors that catalyse ATP-generating reactions. Establishes biochemical vocabulary needed throughout the course.

Lesson 2 • ATP Structure and Chemical Properties

Covers adenosine triphosphate molecular architecture and high-energy phosphate bonds. Connects chemical structure to functional energy release in biological systems.

Lesson 3 • Cellular Energy Currency Concepts

Explains why ATP serves as the universal energy carrier across cell types. Links thermodynamic principles to biological work performance.

Lesson 1 • Energy Payoff Phase and ATP Yield

Analyses steps six through ten where substrate-level phosphorylation generates ATP and NADH. Calculates net ATP gain per glucose molecule.

Lesson 2 • Glucose Uptake and Phosphorylation

Covers glucose transport into cells and initial phosphorylation steps that commit glucose to glycolysis. Establishes the energy investment phase of the pathway.

Lesson 3 • Anaerobic Glycolysis and Lactate Production

Explains lactate dehydrogenase activity and NAD+ regeneration under oxygen-limited conditions. Connects anaerobic glycolysis to muscle fatigue and fermentation applications.

Lesson 4 • Regulation of Glycolytic Flux

Examines allosteric and hormonal controls on glycolytic rate-limiting enzymes. Links flux regulation to cellular energy demand and metabolic disease.

Lesson 1 • Anaplerotic and Amphibolic Functions

Explains how cycle intermediates feed biosynthetic pathways and how anaplerotic reactions replenish them. Demonstrates the cycle's dual catabolic and anabolic roles.

Lesson 2 • Citric Acid Cycle Reactions Five Through Eight

Analyses succinate dehydrogenase through malate dehydrogenase, completing oxaloacetate regeneration. Tallies total electron carriers produced per cycle turn.

Lesson 3 • Energy Accounting and Cycle Regulation

Summarises total electron carrier yield and identifies three key regulatory enzymes. Connects cycle rate to mitochondrial NADH/NAD+ ratio.

Lesson 4 • Pyruvate Dehydrogenase Complex

Details the multienzyme complex converting pyruvate to acetyl-CoA with CO2 release. Establishes the bridge between glycolysis and the citric acid cycle.

Lesson 1 • Total ATP Yield and Efficiency

Calculates theoretical and actual ATP yield per glucose under aerobic conditions. Evaluates metabolic efficiency and factors reducing yield in vivo.

Lesson 2 • Electron Transport Chain Complexes

Details Complexes I through IV, their electron donors, and proton pumping stoichiometry. Connects NADH and FADH2 oxidation to proton gradient formation.

Lesson 3 • ATP Synthase Mechanism

Explains the rotary catalysis mechanism of F0F1-ATP synthase and its subunit organisation. Connects proton flow to conformational changes driving ATP formation.

Lesson 4 • Mitochondrial Structure and Function

Describes inner and outer mitochondrial membrane organisation and its role in ATP synthesis. Establishes the structural basis for chemiosmosis.

Lesson 1 • Beta-Oxidation Reaction Sequence

Details the four repeating steps of beta-oxidation producing acetyl-CoA, NADH, and FADH2. Connects each cycle to progressive chain shortening.

Lesson 2 • Ketone Body Synthesis and Utilization

Describes ketogenesis in the liver during fasting and ketone oxidation in peripheral tissues. Connects ketone bodies to ATP production when glucose is scarce.

Lesson 3 • Unsaturated and Odd-Chain Fatty Acid Oxidation

Explains additional enzymatic steps required for unsaturated and odd-chain fatty acids. Introduces propionyl-CoA metabolism and its connection to the citric acid cycle.

Lesson 4 • ATP Yield from Saturated Fatty Acids

Calculates total ATP from complete oxidation of a saturated fatty acid such as palmitate. Compares energy density of fat versus glucose.

Lesson 1 • Ketogenic Amino Acid Pathways

Identifies amino acids catabolised to acetyl-CoA or acetoacetate, contributing to ketone and lipid synthesis. Distinguishes purely ketogenic from mixed glucogenic-ketogenic amino acids.

Lesson 2 • Nitrogen Removal from Amino Acids

Covers transamination and oxidative deamination reactions that strip nitrogen before carbon catabolism. Links nitrogen disposal to urea cycle inputs.

Lesson 3 • Glucogenic Amino Acid Pathways

Traces carbon skeletons of glucogenic amino acids to pyruvate, oxaloacetate, and other citric acid cycle intermediates. Quantifies their contribution to gluconeogenesis and ATP.

Lesson 4 • Urea Cycle and Nitrogen Excretion

Details the five-step urea cycle, its energetic cost, and its coordination with the citric acid cycle. Establishes the metabolic price of amino acid catabolism.

Lesson 1 • Metabolic Adaptations During Exercise

Traces ATP demand escalation during exercise and the sequential recruitment of phosphocreatine, glycolysis, and oxidative phosphorylation. Quantifies fuel contributions at different intensities.

Lesson 2 • Hormonal Control of Fuel Selection

Examines insulin, glucagon, and epinephrine signalling cascades that shift fuel utilisation between fed and fasted states. Connects hormone action to enzyme phosphorylation and gene expression.

Lesson 3 • AMPK as the Cellular Energy Sensor

Details AMP-activated protein kinase activation by rising AMP/ATP ratio and its downstream targets. Shows how AMPK coordinates catabolic activation and anabolic suppression.

Lesson 4 • Metabolic Adaptations During Fasting and Starvation

Describes the sequential metabolic shifts from glycogen depletion through ketosis to protein catabolism during prolonged fasting. Evaluates organ-specific adaptations preserving brain function.

Lesson 1 • Mitochondrial Dysfunction and ATP Deficits

Examines how mitochondrial mutations, toxins, and ischaemia impair ATP production and cause cellular injury. Connects bioenergetic failure to disease pathophysiology.

Lesson 2 • ATP and Muscle Contraction Mechanics

Explains the cross-bridge cycle, myosin ATPase activity, and calcium-triggered contraction. Connects ATP hydrolysis rate to force generation and fatigue.

Lesson 3 • ATP in Signal Transduction Cascades

Analyses ATP as a phosphate donor in kinase cascades and as an extracellular signalling molecule via purinergic receptors. Links signalling ATP use to cellular responses.

Lesson 4 • ATP-Powered Ion Pumps and Transport

Covers Na+/K+-ATPase, Ca2+-ATPase, and H+-ATPase mechanisms maintaining electrochemical gradients. Quantifies the fraction of cellular ATP consumed by ion transport.