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Phlebotomy Course
More than 2 million students worldwide

Phlebotomy Course

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Launch your healthcare career with a comprehensive Phlebotomy Course that takes you from foundational anatomy to real-world blood collection procedures. You'll master venipuncture technique, infection control, specimen handling, and patient communication — everything you need to work confidently in a clinical setting and pass your national certification exam.

Dedika for Business

What you will learn:

This course covers every core competency required for professional phlebotomy practice. You will study circulatory anatomy, blood composition, and venous site selection to build a strong clinical foundation. You will learn step-by-step procedures for venipuncture and capillary collection, including techniques for neonatal, pediatric, and geriatric patients. Infection control protocols, sharps safety, and biohazard waste management are covered in full detail. You will also gain practical knowledge of specimen processing, laboratory information systems, and quality assurance. The course concludes with certification exam preparation and professional development guidance to help you enter the workforce ready to perform.

How you study in practice Phlebotomy Course

How you practise Phlebotomy Course

For companies looking to train their team

With Dedika for Business, the course includes exercises and examples tailored to your own business and the way your company needs.

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Course Content

8 Chapters • 39 LessonsDuration between 4 and 360 hours (you decide)

Chapter 1See details

Foundations of Phlebotomy Practice

  • Lesson 1 • Medical Terminology for Phlebotomy

    Introduces anatomical and clinical terms used in blood collection settings. Accurate terminology supports documentation, communication, and test ordering.

  • Lesson 2 • Role of the Phlebotomist

    Defines the phlebotomist's scope of practice within the clinical team. Establishes professional boundaries and interdepartmental relationships central to the course.

  • Lesson 3 • Introduction to the Laboratory System

    Explains laboratory departments, specimen routing, and test ordering workflows. Students understand how collected specimens move through the diagnostic process.

  • Lesson 4 • Regulatory and Ethical Framework

    Covers certification requirements, patient rights, and ethical obligations. Connects compliance awareness to safe, lawful practice throughout the course.

Chapter 2See details

Anatomy and Physiology for Blood Collection

  • Lesson 1 • Hemostasis and Coagulation Basics

    Explains the clotting cascade and vascular response to injury. This knowledge underpins post-draw care and recognition of bleeding complications.

  • Lesson 2 • Circulatory System Overview

    Reviews heart function, blood flow pathways, and vessel types. This context supports accurate site selection and complication prevention.

  • Lesson 3 • Alternative Collection Sites

    Introduces veins of the lower extremity, scalp, and external jugular for special populations. Site selection criteria and associated risks are emphasized.

  • Lesson 4 • Blood Composition and Components

    Describes cellular and plasma components and their diagnostic relevance. Understanding composition informs tube selection and specimen handling decisions.

  • Lesson 5 • Venous Anatomy of the Upper Extremity

    Identifies antecubital, forearm, and hand veins used in routine venipuncture. Anatomical landmarks guide needle placement and reduce patient injury.

Chapter 3See details

Infection Control and Safety Protocols

  • Lesson 1 • Sharps Safety and Needlestick Prevention

    Teaches safe needle handling, engineered safety devices, and post-exposure response. Sharps injuries are the leading occupational hazard in phlebotomy.

  • Lesson 2 • Biohazard Waste Management

    Explains classification, segregation, and disposal of biohazardous materials. Correct waste handling prevents environmental contamination and regulatory violations.

  • Lesson 3 • Personal Protective Equipment Selection

    Guides selection, donning, and doffing of gloves, gowns, masks, and eye protection. Proper PPE use prevents cross-contamination during specimen collection.

  • Lesson 4 • Hand Hygiene Techniques

    Covers soap-and-water handwashing and alcohol-based hand rub protocols with correct timing. Hand hygiene is the single most effective infection prevention measure.

  • Lesson 5 • Standard and Transmission-Based Precautions

    Defines standard precautions and isolation categories for airborne, droplet, and contact transmission. Correct precaution selection protects both patient and practitioner.

Chapter 4See details

Phlebotomy Equipment and Supplies

  • Lesson 1 • Capillary Collection Devices

    Covers lancets, microcollection tubes, and filter paper cards used for skin puncture. Device selection is guided by patient age, test type, and blood volume needed.

  • Lesson 2 • Tourniquets and Ancillary Supplies

    Reviews tourniquet types, antiseptic agents, gauze, bandages, and labeling materials. Proper supply preparation reduces procedure time and patient discomfort.

  • Lesson 3 • Venipuncture Needle Systems

    Describes evacuated tube system needles, winged infusion sets, and syringe needles by gauge and length. Needle selection is matched to vein size and patient condition.

  • Lesson 4 • Blood Collection Tubes and Additives

    Identifies tube color codes, additives, and corresponding laboratory tests. Additive knowledge prevents specimen rejection due to incorrect tube selection.

  • Lesson 5 • Order of Draw Principles

    Establishes the standardized sequence for filling multiple tubes to prevent additive carryover. Correct order of draw is essential for result accuracy.

Chapter 5See details

Venipuncture Technique and Procedure

  • Lesson 1 • Needle Insertion and Blood Collection

    Teaches correct needle angle, bevel orientation, and tube engagement during blood draw. Precise technique minimizes hemolysis, hematoma, and patient pain.

  • Lesson 2 • Site Selection and Vein Assessment

    Guides visual inspection and palpation to select the optimal venipuncture site. Systematic assessment reduces failed attempts and patient discomfort.

  • Lesson 3 • Patient Identification and Preparation

    Establishes two-identifier verification, test requisition review, and patient positioning. Correct identification prevents critical misidentification errors.

  • Lesson 4 • Needle Removal and Post-Draw Care

    Covers tourniquet release, needle withdrawal, pressure application, and bandaging. Proper post-draw care prevents hematoma and ensures patient safety.

  • Lesson 5 • Specimen Labeling and Transport

    Defines labeling requirements, transport conditions, and chain-of-custody documentation. Accurate labeling at the bedside is the final safeguard against specimen errors.

Chapter 6See details

Capillary and Special Collection Procedures

  • Lesson 1 • Neonatal and Pediatric Considerations

    Addresses volume limits, developmental communication, and restraint guidelines for young patients. Age-appropriate technique reduces trauma and improves specimen quality.

  • Lesson 2 • Glucose Monitoring and Point-of-Care Testing

    Covers glucometer operation, quality control steps, and result documentation for bedside testing. Point-of-care results directly influence immediate clinical decisions.

  • Lesson 3 • Arterial Blood Gas Collection

    Introduces radial artery puncture, modified Allen test, and specimen handling for ABG analysis. ABG collection requires additional training and competency validation.

  • Lesson 4 • Skin Puncture Technique

    Teaches finger and heel puncture procedures with correct site selection and lancet use. Skin puncture is the preferred method for infants and limited-vein patients.

  • Lesson 5 • Blood Culture Collection

    Details aseptic skin preparation, bottle inoculation sequence, and volume requirements for blood cultures. Contamination prevention is critical for accurate sepsis diagnosis.

Chapter 7See details

Specimen Handling, Processing, and Preanalytical Variables

  • Lesson 1 • Preanalytical Error Sources

    Identifies patient preparation failures, collection errors, and transport problems that alter results. Recognizing error sources is the first step in quality specimen management.

  • Lesson 2 • Specimen Storage and Stability

    Specifies temperature, light, and time requirements for common analytes. Improper storage degrades specimens and generates false laboratory results.

  • Lesson 3 • Specimen Rejection Criteria

    Defines conditions that require specimen rejection and recollection, including clotted, hemolyzed, and mislabeled samples. Rejection criteria protect result accuracy.

  • Lesson 4 • Chain of Custody for Legal Specimens

    Explains documentation, witnessed collection, and tamper-evident sealing for forensic and drug-testing specimens. Chain of custody ensures legal admissibility of results.

  • Lesson 5 • Specimen Processing Procedures

    Covers centrifuge operation, serum and plasma separation, and aliquoting techniques. Correct processing preserves analyte stability and prevents sample rejection.

Chapter 8See details

Complications, Quality Assurance, and Professional Practice

  • Lesson 1 • Quality Assurance in Phlebotomy

    Introduces quality indicators, error tracking, and continuous improvement cycles in specimen collection. QA participation is a professional responsibility for every phlebotomist.

  • Lesson 2 • Difficult Venipuncture Strategies

    Provides techniques for obese, elderly, dehydrated, and oncology patients with challenging veins. Adaptive strategies reduce failed draws and patient distress.

  • Lesson 3 • Documentation and Legal Considerations

    Covers accurate procedure documentation, amendment protocols, and liability awareness. Thorough records protect patients and practitioners in legal and audit contexts.

  • Lesson 4 • Recognizing and Managing Complications

    Identifies hematoma, syncope, nerve injury, and arterial puncture and outlines immediate response steps. Early recognition and correct response minimize patient harm.

  • Lesson 5 • Competency Assessment and Certification Preparation

    Reviews national certification exam domains, practical skills checklists, and continuing education requirements. Certification validates competency and supports career advancement.

Certification

Your valid completion certificate

This course is for you:

  • Career changers: seeking a fast, credentialed entry point into clinical healthcare.

  • Recent high school graduates: ready to build a healthcare career without a four-year degree.

  • Medical assistants: looking to formalize and expand their blood collection competencies.

  • CNA or home health aides: wanting to broaden their clinical scope and job options.

  • Veterans transitioning out: applying discipline and precision to a stable healthcare role.

  • Volunteers or scribes: ready to move from observation into a hands-on patient care position.

What our students say

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