
Carboxytherapy Course
This Carboxytherapy Course gives aesthetic practitioners a complete, clinical-grade foundation in CO2 therapy — from skin anatomy and device operation to advanced body contouring and facial rejuvenation protocols. You'll master injection techniques, patient assessment, complication management, and practice-building strategies. Every module is built for real clinical results.
What you will learn:
You will learn the physiological science behind CO2 therapy, including the Bohr effect, collagen stimulation, and lipolysis mechanisms. The course covers device selection, calibration, and maintenance so you can run a safe, efficient treatment station. You will develop systematic patient assessment and protocol design skills for both facial and body indications. Injection mapping, flow rate control, and sterile technique are trained in detail across multiple treatment areas. You will also gain the tools to manage complications, document outcomes, and grow a profitable carboxytherapy practice.
How you study in practice Carboxytherapy Course
How you practise Carboxytherapy Course
For companies looking to train their team
With Dedika for Business, the course includes exercises and examples tailored to your own business and the way your company needs.
Course Content
8 Chapters • 40 LessonsDuration between 4 and 360 hours (you decide)
Chapter 1HideHide detailsSee detailsFoundations of Carboxytherapy Science
Foundations of Carboxytherapy Science
Lesson 1 • Regulatory and Safety Framework
Outlines medical-grade CO2 standards, device certification requirements, and scope-of-practice boundaries. Prepares students for compliant clinical operation.
Lesson 2 • CO2 Physiology and the Bohr Effect
Explains how CO2 shifts oxygen dissociation curves and drives vasodilation. Links biochemistry directly to observed clinical outcomes.
Lesson 3 • Skin Anatomy Relevant to Treatment
Reviews epidermis, dermis, and subcutaneous layers targeted during carboxytherapy. Grounds technique decisions in anatomical reality.
Lesson 4 • History and Evolution of CO2 Therapy
Traces carboxytherapy from spa origins to modern clinical use. Provides context for current protocols and regulatory acceptance.
Lesson 5 • Mechanisms of Therapeutic Action
Details collagen stimulation, lipolysis, and microcirculation enhancement triggered by CO2. Connects mechanisms to specific treatment indications.
Chapter 2HideHide detailsSee detailsEquipment, Devices, and CO2 Gas
Equipment, Devices, and CO2 Gas
Lesson 1 • Equipment Maintenance and Troubleshooting
Establishes routine maintenance schedules and common fault-resolution procedures. Minimizes downtime and sustains treatment quality.
Lesson 2 • CO2 Gas Cylinders and Supply Chain
Covers medical-grade cylinder procurement, storage, and handling protocols. Ensures uninterrupted, safe gas supply in clinical practice.
Lesson 3 • Needles, Tubing, and Consumables
Identifies correct needle gauges, tubing specifications, and single-use consumable standards. Supports sterile technique and consistent gas delivery.
Lesson 4 • Device Setup and Calibration
Walks through pre-treatment device assembly, flow-rate calibration, and pressure verification. Directly prevents equipment-related adverse events.
Lesson 5 • Types of Carboxytherapy Devices
Compares needle-based, transdermal, and combined delivery systems. Enables informed device selection based on indication and setting.
Chapter 3HideHide detailsSee detailsPatient Assessment and Treatment Planning
Patient Assessment and Treatment Planning
Lesson 1 • Informed Consent and Pre-Treatment Prep
Guides practitioners through consent documentation and pre-treatment skin preparation steps. Establishes legal and clinical readiness before each session.
Lesson 2 • Comprehensive Patient Consultation
Structures the intake interview to capture medical history, aesthetic goals, and expectations. Forms the foundation for safe, personalized treatment.
Lesson 3 • Designing the Treatment Protocol
Translates assessment findings into a structured protocol specifying flow rate, volume, and session count. Ensures reproducible, goal-aligned outcomes.
Lesson 4 • Skin and Body Analysis Techniques
Applies visual, tactile, and device-assisted analysis to characterize treatment areas. Informs injection depth, volume, and session frequency decisions.
Lesson 5 • Contraindications and Risk Stratification
Identifies absolute and relative contraindications and stratifies patient risk levels. Prevents adverse events through systematic pre-treatment screening.
Chapter 4HideHide detailsSee detailsCore Injection Techniques and Protocols
Core Injection Techniques and Protocols
Lesson 1 • Intradermal Injection Technique
Teaches correct needle angle, depth, and bevel orientation for intradermal CO2 delivery. Targets superficial skin rejuvenation and scar treatment indications.
Lesson 2 • Flow Rate and Volume Management
Trains precise control of CO2 flow rate and total volume per session and per point. Directly determines treatment efficacy and patient comfort.
Lesson 3 • Subcutaneous Injection Technique
Develops skill in deeper subcutaneous CO2 delivery for fat reduction and cellulite. Builds on intradermal technique with adjusted depth and volume parameters.
Lesson 4 • Sterile Field and Injection Preparation
Establishes aseptic workspace setup and pre-injection skin disinfection standards. Prevents infection and cross-contamination at every session.
Lesson 5 • Injection Mapping for Body Regions
Applies standardized injection maps to abdomen, thighs, arms, and periorbital areas. Ensures systematic coverage and reproducible session-to-session results.
Chapter 5HideHide detailsSee detailsFacial Carboxytherapy Applications
Facial Carboxytherapy Applications
Lesson 1 • Skin Rejuvenation and Texture Improvement
Uses intradermal CO2 to stimulate collagen and improve overall skin tone and texture. Builds directly on intradermal technique from core injection training.
Lesson 2 • Periorbital Dark Circle Treatment
Delivers targeted CO2 injections to improve periorbital microcirculation and reduce pigmentation. Addresses the most requested facial carboxytherapy indication.
Lesson 3 • Scar and Stretch Mark Treatment on the Face
Adapts CO2 injection parameters for atrophic acne scars and superficial facial scars. Requires precise depth control to remodel dermal architecture.
Lesson 4 • Post-Treatment Facial Care Protocols
Specifies immediate aftercare, product restrictions, and follow-up scheduling for facial sessions. Protects results and reduces adverse reaction risk.
Lesson 5 • Facial Anatomy and Injection Safety Zones
Maps facial vascular danger zones and safe injection corridors for CO2 delivery. Prevents vascular complications unique to the face.
Chapter 6HideHide detailsSee detailsBody Contouring and Cellulite Reduction
Body Contouring and Cellulite Reduction
Lesson 1 • Cellulite Pathophysiology and Grading
Reviews the structural causes of cellulite and applies standardized grading scales. Grounds protocol selection in objective clinical assessment.
Lesson 2 • Combining Carboxytherapy with Body Treatments
Integrates CO2 therapy with complementary modalities such as ultrasound and lymphatic drainage. Maximizes outcomes through evidence-based combination sequencing.
Lesson 3 • Stretch Mark Treatment on the Body
Adapts CO2 parameters to stimulate collagen in striae distensae on abdomen and thighs. Addresses a high-demand indication with specific depth and volume adjustments.
Lesson 4 • Cellulite-Specific Injection Strategies
Applies superficial subcutaneous CO2 to disrupt fibrous septa and improve skin texture. Requires differentiated technique from general fat reduction protocols.
Lesson 5 • Subcutaneous Fat Reduction Protocol
Delivers high-volume subcutaneous CO2 to promote lipolysis in localized fat deposits. Builds on subcutaneous injection technique with body-specific parameters.
Chapter 7HideHide detailsSee detailsManaging Adverse Events and Complications
Managing Adverse Events and Complications
Lesson 1 • Common Side Effects and Normal Responses
Distinguishes expected post-treatment responses from true adverse events. Enables accurate patient communication and appropriate clinical decision-making.
Lesson 2 • Gas Embolism: Recognition and Response
Covers the rare but critical risk of gas embolism, its presentation, and emergency response. Prepares practitioners for the most severe carboxytherapy complication.
Lesson 3 • Vascular and Neurological Complications
Identifies signs of vascular occlusion and nerve irritation specific to CO2 injection. Requires immediate recognition and escalation to prevent lasting harm.
Lesson 4 • Adverse Event Reporting and Quality Review
Establishes structured reporting workflows and root-cause analysis for adverse events. Drives continuous safety improvement across the practice.
Lesson 5 • Infection Prevention and Management
Applies strict aseptic protocols and identifies early signs of injection-site infection. Prevents the most common serious complication of needle-based procedures.
Chapter 8HideHide detailsSee detailsAdvanced Protocols and Practice Optimization
Advanced Protocols and Practice Optimization
Lesson 1 • Advanced Combination Treatment Design
Designs multi-modality protocols combining CO2 with injectables, energy devices, and topicals. Requires mastery of all prior technique and safety chapters.
Lesson 2 • Outcome Measurement and Clinical Photography
Applies standardized photography, validated scales, and patient-reported measures to track results. Enables evidence-based protocol refinement and marketing credibility.
Lesson 3 • Building a Quality Assurance System
Implements clinical audits, peer review, and continuing education frameworks for the practice. Sustains high standards as the field and technology evolve.
Lesson 4 • Difficult Cases and Special Populations
Adapts protocols for patients with darker skin tones, obesity, or chronic conditions. Expands clinical range while maintaining safety standards.
Lesson 5 • Maintenance and Long-Term Treatment Planning
Designs maintenance schedules to sustain results after the initial treatment course. Builds patient retention and long-term practice revenue.
Your valid completion certificate
This course is for you:
Licensed aesthetician: ready to add a clinically credible CO2 service to their menu.
Aesthetic nurse: seeking structured protocols to expand beyond injectables into CO2 therapy.
Beauty therapist: transitioning from spa services into results-driven clinical treatments.
Dermatology clinic staff: wanting in-house carboxytherapy competency for existing patients.
Independent clinic owner: looking to differentiate their practice with a specialized offering.
Career changer: entering the aesthetic field with a focus on body contouring treatments.
What our students say
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