Bio Tech Course

Lesson 1 • Essential Cell Biology Review

Reviews prokaryotic and eukaryotic cell structure relevant to biotech applications. Anchors molecular techniques to cellular context.

Lesson 2 • Biochemistry Essentials for Biotech

Covers proteins, lipids, carbohydrates, and nucleic acids as substrates and products in biotech workflows. Builds chemical literacy needed for lab techniques.

Lesson 3 • Biotech Industry and Career Pathways

Maps major industry sectors—pharma, agriculture, diagnostics, and industrial biotech—to job roles. Orients students to professional expectations.

Lesson 4 • History and Scope of Biotechnology

Traces biotechnology from ancient fermentation to modern genomics. Provides context for why each subsequent technique was developed.

Lesson 1 • Lab Safety and Good Practice

Establishes biosafety levels, personal protective equipment, and waste disposal standards. Ensures safe execution of all subsequent lab work.

Lesson 2 • Gel Electrophoresis Techniques

Covers agarose and polyacrylamide gel systems for separating nucleic acids and proteins. Builds analytical skills for verifying molecular biology experiments.

Lesson 3 • Spectrophotometry and Quantification

Teaches UV-Vis and fluorescence spectrophotometry for quantifying nucleic acids and proteins. Provides measurement skills used in nearly every biotech workflow.

Lesson 4 • Centrifugation and Separation Methods

Covers differential and density-gradient centrifugation for isolating cells, organelles, and macromolecules. Links separation principles to purification workflows.

Lesson 1 • DNA Sequencing Methods

Introduces Sanger sequencing and next-generation sequencing platforms for verifying constructs and profiling genomes. Sequence verification is mandatory before downstream use.

Lesson 2 • Restriction Enzymes and DNA Cloning

Covers restriction digestion, ligation, and vector selection for inserting genes into expression systems. Cloning skills enable production of recombinant proteins and constructs.

Lesson 3 • Polymerase Chain Reaction

Explains PCR thermocycling, primer design, and variant techniques such as RT-PCR and qPCR. Amplification underpins cloning, diagnostics, and expression analysis.

Lesson 4 • Nucleic Acid Extraction and Purification

Details protocols for isolating genomic DNA, plasmid DNA, and RNA from diverse sample types. Clean nucleic acids are prerequisite for all downstream molecular work.

Lesson 1 • Fermentation and Cell Culture Scale-Up

Teaches batch, fed-batch, and perfusion culture modes for scaling protein production. Process parameters control yield and product quality.

Lesson 2 • Protein Purification Strategies

Details affinity, ion-exchange, and size-exclusion chromatography steps for isolating target proteins. Purification trains the multi-step thinking needed in downstream processing.

Lesson 3 • Expression System Selection

Compares bacterial, yeast, insect, and mammalian expression hosts by yield, folding, and post-translational modification needs. Host choice determines downstream purification strategy.

Lesson 4 • Protein Characterization and Quality

Applies Western blotting, mass spectrometry, and activity assays to confirm protein identity and function. Characterization data supports regulatory submissions.

Lesson 1 • Ethical and Regulatory Considerations

Examines biosafety guidelines, informed consent, and germline editing debates governing genome editing research. Regulatory literacy prevents compliance failures in applied projects.

Lesson 2 • Editing Verification and Off-Target Analysis

Applies T7E1 assay, Sanger sequencing, and whole-genome sequencing to confirm edits and detect off-target sites. Verification is required before therapeutic or commercial use.

Lesson 3 • Principles of Genome Editing

Explains double-strand break repair pathways—NHEJ and HDR—as the mechanistic basis for all editing tools. Understanding repair determines which editing strategy to choose.

Lesson 4 • CRISPR-Cas9 System Design

Covers guide RNA design, PAM site selection, and Cas9 delivery methods for efficient editing. Proper design minimizes off-target effects and maximizes editing efficiency.

Lesson 1 • Microbial and Cell Growth Kinetics

Applies Monod kinetics and logistic growth models to predict and optimize culture performance. Kinetic modeling links biological behavior to engineering control strategies.

Lesson 2 • Process Analytical Technology

Introduces inline and at-line sensors, multivariate data analysis, and real-time release testing for process control. PAT reduces batch failures and supports continuous manufacturing.

Lesson 3 • Mass Transfer and Oxygen Delivery

Explains volumetric oxygen transfer coefficient and agitation strategies for maintaining aerobic cultures. Oxygen limitation is a primary cause of yield loss at scale.

Lesson 4 • Bioreactor Design and Operation

Covers stirred-tank, airlift, and hollow-fiber bioreactor configurations and their operating parameters. Reactor choice shapes mixing, oxygen transfer, and shear stress on cells.

Lesson 1 • Biologics Drug Discovery

Covers target identification, lead molecule selection, and early feasibility studies for biologic candidates. Discovery decisions determine the entire downstream development path.

Lesson 2 • Clinical Trial Phases and Design

Explains Phase I–III trial objectives, endpoints, and adaptive design principles for biologics. Trial design directly affects approval timelines and data quality.

Lesson 3 • Regulatory Pathways for Biologics

Navigates biologics license applications, biosimilar approval, and accelerated designation programs. Regulatory strategy determines market access speed and commercial success.

Lesson 4 • Preclinical Development and Testing

Details in vitro and in vivo safety, pharmacokinetics, and efficacy studies required before human trials. Preclinical data packages support regulatory filing for clinical entry.

Lesson 1 • Regulatory Inspections and Audits

Prepares teams for regulatory agency inspections and internal audits through mock audit practice. Inspection readiness protects manufacturing licenses and product supply.

Lesson 2 • Process Validation and Qualification

Guides installation, operational, and performance qualification of equipment and processes. Validation data demonstrates consistent product quality to regulators.

Lesson 3 • Good Manufacturing Practice Principles

Covers facility design, personnel hygiene, and batch record requirements mandated by GMP regulations. GMP compliance is non-negotiable for commercial biologic manufacturing.

Lesson 4 • Quality Management Systems

Establishes document control, change management, and CAPA systems as the backbone of biotech quality. A robust QMS prevents deviations and supports regulatory inspections.