Papers in Molecular Genetics Course

Lesson 1 • Mendelian and Molecular Inheritance

Links classical segregation laws to allele-level molecular mechanisms. Provides the inheritance framework required to evaluate genetic mapping studies.

Lesson 2 • Regulation of Gene Expression

Introduces transcriptional, post-transcriptional, and epigenetic control mechanisms. Grounds students in regulatory logic used throughout experimental genetics literature.

Lesson 3 • Central Dogma and Gene Expression

Explains transcription, translation, and RNA processing. Connects molecular flow of information to phenotypic outcomes discussed in research papers.

Lesson 4 • Mutation Types and Consequences

Catalogues point mutations, indels, and chromosomal rearrangements with functional effects. Prepares students to assess mutant phenotypes reported in papers.

Lesson 1 • Anatomy of a Research Article

Maps the IMRaD format and explains each section's rhetorical function. Gives students a consistent framework for rapid paper navigation.

Lesson 2 • Tracing Citations and Context

Explains how to follow citation chains to verify claims and locate foundational studies. Situates individual papers within the broader research conversation.

Lesson 3 • Identifying Hypotheses and Aims

Teaches extraction of explicit and implicit research questions from introductions. Connects stated aims to experimental design choices made in the methods.

Lesson 4 • Interpreting Figures and Tables

Builds skills for reading gel images, graphs, and statistical tables independently of author interpretation. Directly supports critical evaluation of data quality.

Lesson 1 • Cloning and Recombinant DNA Methods

Reviews restriction enzyme cloning, ligation, and vector design. Provides the conceptual basis for evaluating construct validation in published studies.

Lesson 2 • Gel Electrophoresis and Blotting

Details agarose and polyacrylamide separation, Southern, Northern, and Western blotting. Connects band patterns to molecular weight and abundance interpretations.

Lesson 3 • Nucleic Acid Isolation and Quantification

Covers DNA and RNA extraction logic, purity assessment, and storage. Establishes quality-control benchmarks referenced throughout experimental papers.

Lesson 4 • PCR and Its Variants

Explains PCR thermocycling logic, primer design, and quantitative applications. Prepares students to assess amplification controls and efficiency data in papers.

Lesson 1 • Sanger Sequencing Principles

Explains dideoxy chain termination, capillary electrophoresis, and read interpretation. Provides the baseline sequencing standard against which newer methods are compared.

Lesson 2 • Genome Assembly and Annotation

Covers de novo assembly, reference-guided mapping, and gene annotation pipelines. Connects assembly quality metrics to downstream analysis reliability.

Lesson 3 • Variant Calling and Interpretation

Explains SNP, indel, and structural variant detection from sequencing data. Prepares students to evaluate variant filtering criteria and pathogenicity claims.

Lesson 4 • Next-Generation Sequencing Platforms

Compares short-read and long-read sequencing chemistries and their trade-offs. Equips students to judge platform choice relative to the biological question asked.

Lesson 1 • CRISPR-Cas9 Mechanism and Design

Explains guide RNA targeting, Cas9 cleavage, and repair pathway outcomes. Provides the mechanistic foundation for evaluating editing experiments in papers.

Lesson 2 • CRISPR Variants and Base Editing

Covers CRISPRi, CRISPRa, base editors, and prime editing systems. Enables students to match the editing tool to the experimental goal described in papers.

Lesson 3 • Off-Target Detection and Validation

Reviews GUIDE-seq, Digenome-seq, and amplicon sequencing for off-target profiling. Equips students to assess whether off-target controls are adequate in published studies.

Lesson 4 • RNAi and Knockdown Approaches

Explains siRNA, shRNA, and antisense oligonucleotide mechanisms and controls. Prepares students to evaluate knockdown efficiency and specificity in papers.

Lesson 1 • ChIP-seq for Protein-DNA Interactions

Details chromatin immunoprecipitation, peak calling, and motif enrichment analysis. Prepares students to assess antibody specificity and input controls in ChIP-seq papers.

Lesson 2 • RNA-seq Experimental Design

Covers library preparation choices, replication strategy, and batch effect management. Connects design decisions to the statistical power of differential expression results.

Lesson 3 • Single-Cell Transcriptomics

Covers droplet-based scRNA-seq, cell clustering, and trajectory inference. Enables assessment of cell-type annotation and dimensionality reduction choices in papers.

Lesson 4 • Differential Gene Expression Analysis

Explains read alignment, count normalisation, and DESeq2/edgeR statistical models. Enables evaluation of fold-change thresholds and adjusted p-value reporting in papers.

Lesson 1 • Phylogenetics and Molecular Evolution

Covers tree-building methods, substitution models, and molecular clock analysis. Enables critical reading of phylogenomic and comparative genomics papers.

Lesson 2 • Hardy-Weinberg Equilibrium and Allele Frequencies

Reviews HWE assumptions, deviations, and their implications for genotyping quality. Provides the statistical baseline for evaluating GWAS and population studies.

Lesson 3 • Linkage Disequilibrium and Haplotypes

Explains LD metrics, haplotype blocks, and tag SNP selection. Connects LD structure to the interpretation of association signals in GWAS papers.

Lesson 4 • Genome-Wide Association Studies

Covers GWAS design, Manhattan plots, and fine-mapping strategies. Enables evaluation of significance thresholds, replication, and functional follow-up in papers.

Lesson 1 • Systematic Literature Review Methods

Covers PRISMA guidelines, search strategy design, and inclusion criteria development. Enables students to conduct reproducible, transparent reviews of genetics topics.

Lesson 2 • Synthesising Evidence and Identifying Gaps

Teaches evidence hierarchy, conflicting result reconciliation, and gap identification. Culminates the course by enabling students to propose well-grounded future research directions.

Lesson 3 • Assessing Experimental Rigor and Reproducibility

Applies criteria for evaluating controls, sample sizes, and independent replication. Directly synthesises technical knowledge from all prior chapters into a unified appraisal framework.

Lesson 4 • Identifying Logical Fallacies in Papers

Catalogues common reasoning errors including correlation-causation conflation and overgeneralisation. Sharpens students' ability to distinguish robust conclusions from overreach.